Materials Included in the KITALYSIS-RCM-2PAK High-Throughput Screening Kit

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Medium (5,6,7) Ring Closing Metathesis

Contents in each of the 2 individually sealed Mylar (foil bags):

  • 24 (6 x 4) pre-weighed catalysts in glass vials loaded with stir bars, topped with cap mat.
  • 4 empty reaction vials with lids (to make stock solutions)

The screening sets come pre-loaded with 1 µmol of catalyst in each vial according to the following design.

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Description Product No.   Vial
Grubbs Catalyst™ 2nd Generation
A1, B1, C1, D1
Hoveyda-Grubbs Catalyst™ 2nd Generation 569755 A2, B2, C2, D2
Grubbs Catalyst™ C571 682373 A3, B3, C3, D3
Grubbs Catalyst™ C711 729345 A4, B4, C4, D4
Grubbs Catalyst™ 1st Generation 579726 A5, B5, C5, D5
Grubbs Catalyst™ C827 682365 A6, B6, C6, D6

All contents in the foil bag are weighed, plated, packed, and sealed in a glove box under nitrogen.

Ampule Boxes:

  • Chlorobenzene (ClPh) : 4 x 2 mL of degassed, anhydrous Sure/Seal™
  • Toluene: 4 x 2 mL of degassed, anhydrous Sure/Seal™
  • Isopropyl acetate (i-PrOAc): 4 x 2 mL of degassed, anhydrous Sure/Seal™
  • Methyl THF (Me-THF): 4 x 2 mL of degassed, anhydrous Sure/Seal™
  • TFA (neat):  2 x 2 mL degassed, anhydrous Sure/Seal™

Internal Standard:

  • Biphenyl (30 mg). To be added to the reaction during the work-up. Recipe for the work-up with the internal standard can be found within the hyperlinked screen types in the "Kit Design" section below.

KitAlysis 24-well Reaction Block Replacement Films-2EA

  • Pack of 2 enables a new film to be used with each kit ensuring a tight, cross-contaminate-free seal every time.

Stir Bars-8 individually packed

  • Fit perfectly into supplied substrate vials to ensure proper stirring of substrate mixtures prior to dosing.

Kit Design

The Medium Ring Closing Metathesis kit was designed to provide the best possible chance of finding good reaction conditions to close 5, 6, and 7-membered rings and is run with:

  • 20 µmol of your RCM substrate
  • 1 µmol catalyst per vial; 6 in this system. 5 mol% per reaction.
  • 0.2M solvent concentration (ClPh, Toluene, i-PrOAc, and Me-THF)
  • TFA neat. Optional additive. See kit step-by-step user guide for details and guide on when to use.
  • 60 °C (Recommended). Temperatures not to exceed 80 °C. Lower temperatures may also be optional but 60 °C is an excellent overall starting point.

The step-by-step user guides provide comprehensive instructions for using the kits and an excel sheet for all stock solution recipes.

Use of the Provided Tools

Multiple tools have been created to ensure your success with kit set up. Start with the more detailed guide to ensure you are comfortable with all of the steps before using the quick guides on the excel worksheet. Remember that while the technique is new, it is still organic chemistry and so the steps will seem easy once you try just one kit. It is just a new way of approaching something you are already very good at.

Detailed Set-Up User Guide:

The step-by-step user guide is designed for the first-time user and should be read completely before getting started; and used in conjunction with the video. This guide includes trouble-shooting tips, how-to’s for the Labware, and work-up recipes with procedures. Everything you need to set up a kit with confidence every time.

Excel Sheet:

The downloadable excel files are specific to the kit being run and can be found within each Step-by-Step User Guide. They have the following features:

  • Calculations for substrate recipes depending upon the molecular weight of your substrates
  • Quick directions for the more avid user
  • Print button to allow you to take recipe to the lab
  • Contains all info such that they can be can be “printed” (saved as a pdf and appended to ELN for experimental information