UHPLC (Isocratic) Analysis of 14 Cannabinoids on Chromolith® HR RP-18e

UHPLC (Isocratic) Analysis of 14 Cannabinoids on Chromolith® HR RP-18e


instrument Dionex/Thermo Electron Ultimate 3000 HPLC
column Chromolith HR RP-18e, 10 cm x 4.6 mm (1.52022)
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
isocratic 30/70 [A]/[B]
flow rate 1.5 mL/min
pressure 1100 psi (75,8 bar)
column temp. 30°C
detector UV at 230nm
injection 5µL
sample 25µg/mL 14 Cannabinoid mix in acetonitrile


Analysis Note Cannabinoids are a group of active compounds found in cannabis. They reportedly provide relief to an assortment of symptoms in the treatment of pain, nausea, anxiety, mood disorders, and inflammatory diseases. Cannabinoids imitate naturally occurring compounds and interact with two specific types of receptors: CB1 and CB2, located in the nervous system and immune system, respectively. Shown here is an isocratic separation of fourteen cannabinoids in less than ten minutes on a Chromolith HR RP-18e column. Cerilliant cannabinoid CRMs provide reliable identification and quantification. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing.
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany


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