CHOZN® Products and Services

Biopharmaceutical Expression Systems

Production Lines, Media and Feeds Optimized for the Demands of Biopharmaceutical Manufacturing

SAFC offers multiple levels of support for your biopharmaceutical expression systems needs with a focus on increasing production titer and decreasing development timelines. In addition to our long history of reliability in supplying high grade critical raw materials, our offerings are backed by top notch technical support by Ph.D. level scientists and comprehensive protocols for ease of use. Decrease your time to market and costs by leveraging the CHOZN manufacturing system!

CHOZN Platform Cell Line Engineering
and Development
CHOZN Cell Lines CHOZN Media and
Feed Systems
CompoZr ZFNs
• GS -/- cell line
• Expression vector
• Optimized cGMP
  media and feed
• Technical support
• Recombinant cell line
• Gene knockout
• Targeted integration
• CHO GS -/-
• CHO DHFR -/-
• EX-CELL Advanced
  Medium and feed
• CD CHO Fusion
• CHO Cloning Medium
• Custom ZFNs
  engineered to any
  gene target

CHOZN Platform

The CHOZN® Platform is a CHO (Chinese Hamster Ovary) mammalian cell expression system for the fast and easy selection and scale up of clones producing high levels of recombinant proteins. Key to the CHOZN® Platform is the development of the CHOZN® ZFN Modified GS-/- CHO cell line that eliminates the endogenous Glutamine synthetase (GS). The CHOZN® Platform also includes an optimized set of cGMP produced chemically defined (CD) growth and production media and feed that have been developed to maximize the growth and production of r-proteins from producing clones. 

Cell Line Engineering and Development

The CHOZN Platform Development Team at SAFC leverages several molecular biology technologies, most notably CompoZr® Zinc Finger Nuclease Technology, to engineer mammalian lines for customers to use in bioproduction. Our mission is to facilitate customer’s research and manufacturing needs through application of our experience in cell engineering technologies. We can also perform custom cell line development of recombinant cell lines in our CHOZN GS CHO Cell Line. Alternatively, we can perform the work in your host cell line. 

CHOZN Cell Lines

SAFC has created a series of zinc finger nuclease (ZFN) modified CHO cell lines with characteristics that are attractive in a platform cell line to biopharmaceutical developers and manufacturers. 

CHOZN Media and Feed System

SAFC's new EX-CELL Advanced media and feed system was designed and optimized for the CHOZN GS cell line using multiple molecules, including mAbs and fusion proteins, resulting in 3-5 fold increases in therapeutic productivity while maintaining critical protein attributes. The EX-CELL Advanced system has been shown to support in excess of 5g/L in CHOZN platform cell line.

CompoZr Zinc Finger Nucleases

Zinc Finger Nucleases (ZFN) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. ZFN technology allows for easy creation of novel cell lines, including CHO, with precise and heritable gene additions, deletions or modifications.

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Learn more about the CHOZN Expression Platform
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