Webinars



 Upcoming and previously recorded webinars

Webinar: “Understanding the Big “Ome” – A Wide Lens Perspective of The Human Protein Atlas (HPA) Program


 Abstract

In the last decade, advances in technologies and informatics have been used to generate and process large biological data sets (commonly referred to as “omics data”).  The availability of such data is promoting a critical shift in the study of biomedical sciences. Genomics, transcriptomics and proteomics, coupled with bioinformatics and biostatistics, are gaining momentum.

However, for the most part, these technologies are assessed individually with distinct approaches generating monothematic rather than integrated knowledge.

As other areas of biomedical sciences, including metabolomics, epigenomics and pharmacogenomics, are moving towards the omics scale, we are witnessing the rise of inter-disciplinary data integration strategies to support a better understanding of biological systems and eventually the development of successful precision medicine.

The Human Protein Atlas

Introduction

The Human Protein Atlas is a Swedish-based program initiated in 2003 with the aim to map all the human proteins in cells, tissues and organs using integration of various omics technologies, including antibody-based imaging, mass spectrometry-based proteomics, transcriptomics and systems biology.

All the data in the knowledge resource is open access to allow scientists both in academia and industry to freely access the data for exploration of the human proteome. The Human Protein Atlas consists of six separate subparts, each focusing on a particular aspect of the genome-wide analysis of the human proteins; the Tissue Atlas showing the distribution of the proteins across all major tissues and organs in the human body, the Cell Atlas showing the subcellular localization of proteins in single cells, and the Pathology Atlas showing the impact of protein levels for survival of patients with cancer. Newly added components include the Blood, Brain and Metabolic Atlases. Significant findings from the atlas are discussed.

India is a major contributor to both the Tissue and Pathology Atlas for The Human Protein Atlas (HPA) Program.

 

Speaker Bio
Dr Sanjay Navani
Surgical Pathologist
Lab Surgpath
Dr. Sanjay Navani, Consultant Surgical Pathologist and Immunohistochemist, provides surgical pathology services through his laboratory –LAB SURGPATH. Lab Surgpath is currently the only laboratory in the country that offers an Immunohistochemistry Stained Slide Service for more than 200 diagnostic and 20000 research antibodies.

Lab Surgpath pathologists have contributed to The Human Protein Atlas (HPA) program by manually annotating approximately 16 million immunohistochemistry images over a period of eight years. The immunohistochemistry images form the backbone of the protein atlas and are available for viewing at www.proteinatlas.org.

This research effort from India is the largest effort in evaluation of immunohistochemistry antibodies numbering approximately 25000.

Dr Navani finished his MD (Pathology) from Kasturba Medical College, Mangalore University in 1992. Subsequently he obtained Fellowship in Gynecologic Pathology, 1995-96 at Massachusetts General Hospital, Harvard Medical School, Boston, USA under Dr Robert Young and Dr Robert Scully.

Dr Navani set up the Immunohistochemistry Section at the Department of Surgical Pathology and Cytology at the Breach Candy Hospital Trust in 1995. He has served as Surgical Pathologist and Immunohistochemist at Breach Candy Hospital Trust and Indian Cancer Society from 1995 to 2007.

Webinar: Making the Switch to Synthetic Chemistry in Water - Faster, Better, Cheaper & Environmentally Responsible



 Abstract

This presentation focuses on developing alternative technologies that address three key parameters associated with research today in organic synthesis, with a heavy emphasis on green chemistry. Major areas of research include:

  • Synthesis and use of new ligands for Pd-catalyzed Suzuki-Miyaura couplings that can be effected in water under mild conditions and at the 1000 ppm level of metal invested
  • Preparation and applications of novel nanoparticles containing ppm levels of Pd
  • Developing new technologies for Sonogashira couplings in water that require <1000 ppm loadings of Pd, enabled by aqueous micellar catalysis
Speaker Bio
Bruce H. Lipshutz, Ph.D.
Distinguished Professor
University of California, Santa Barbara
Areas of Specialization include: Organic & Bioorganic, Molecular Design & Synthesis, Biomedical Sciences, Catalysis & Green Chemistry

Webinar: Genome Engineering to Synthetic Genomics

30 Year Research Odyssey of an Indian Born American Scientist

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 Abstract

Over the 30 years of my scientific research career at the Johns Hopkins Medical Institutions, I have been very fortunate to work on two exciting areas in life sciences: 1) genome engineering (aka genome editing) and 2) synthetic biology (more specifically synthetic genomics). Our basic research on FokI restriction enzyme, led to the creation of zinc finger nucleases (ZFNs), which were the first truly targetable reagents that showed pre-determined DNA sequences could be addressed for cleavage by protein engineering [1-3]. Our studies on the mechanism of cleavage by 3-finger ZFNs established that the preferred substrates were paired binding sites, which doubled the size of the target sequence recognition from 9 to 18 bp, long enough to specify a unique genomic locus in plant and mammalian cells [4]. Soon afterwards, we showed that a ZFN-induced targeted DSB stimulates homologous recombination in cells, using frog oocytes as a model system [5]. Thus, ZFNs ushered in the era of genome editing.

Read more

 

Speaker Bio
Srinivasan Chandrasegaran
Professor
Department of Environmental Health and Engineering
Johns Hopkins School of Public Health
Baltimore, Maryland 21205, USA
Srinivasan Chandrasegaran Ph.D. is a tenured Professor in the Department of Environmental Health and Engineering at the Johns Hopkins School of Public Health (JHSPH). His lab reported the creation of designer zinc finger nucleases (ZFNs) in 1996. He then showed in 2001 the utility of ZFNs in stimulating site-specific recombination in frog oocytes in a collaboration with Dana Carroll lab. His lab was the first to report the total synthesis of a functional designer eukaryotic (yeast) chromosome, synIII, in 2014.

Webinar: Journey of Protein from Extraction to Purification

30 October 2017
 

 Abstract

Various methods for the extraction and purification of recombinant proteins from bacteria are in use and have been well described. Common methods for cell lysis involve mechanical disaggregation of cells, such as sonication, but this can be time-consuming when working with multiple samples and more importantly can result in localized heating leading to loss of protein activity. In this seminar, we provide data showing that gentle detergent based lysis leads to greater protein activity and higher yields when compared to mechanical methods such as sonication. We also investigate the use of dual purification strategies and explore optimizing conditions to improve the purity of protein purification techniques.

 

Speaker Bio
Dr. Natasha L. Pirman
Application Scientist for Molecular Workflow Tools
Dr. Natasha L. Pirman is an Application Scientist for Molecular Workflow Tools. She is involved in developing new sample preparation products and applications for both protein and nucleic acid applications. Prior in her career she uilized her expertise in protein biochemistry to explore structural protein changes to Intrinsically Disordered Proteins and human kinase disease models for drug target investigations.


 

Webinar: Trusted Tools and Innovation in General and Specialty Cell Culture

15 November 2017
 

 Abstract

Cell culturing represents a fundamental research tool for life scientists by offering a unique ability to grow, manipulate, and analyze populations of cells.  From experiments utilizing the latest gene editing technologies to simple growth assays, high quality tools to ensure reproducible and reliable cell culturing is critical for the success of any life science projects. In this webinar, key considerations for choosing the right tools for various aspects of cell culturing will be presented. Starting from sterile filtration and cell enumeration through micro-porous membrane based functional assays and live cell imaging, audiences will be able to gain insights into parameters affecting cell culturing such as the choice of membranes and pore sizes and also learn about innovative microfluidics based tools for cell enumeration and culturing.

 

Speaker Bio
Jun Park, Ph. D.
Senior Applications Scientist
Jun Park, Ph. D. is Senior Applications Scientist. He studied Biomaterials and Immunology at University of Toronto and did his postdoctoral work at Harvard Medical School investigating small molecule regulators of beta integrins. Since 2006 his primary works have focused on the novel detection technologies, Western blotting, and cell based assays.      

Webinar: Not All Spots are Created Equal: Maximizing ELISpot Data Quality through Workflow Optimization

12 December 2017
 

 Abstract

Precise regulation of lymphocyte function is critical to mounting a specific immune response; this complex process requires exquisite control of processes regulating expression of effector capabilities. Understanding such mechanisms is essential for the development of therapeutics that enhance endogenous immune function. While no one assay can measure all parameters, the ELISpot offers quantitative assessment of effector function(s) at the single cell level with superior sensitivity. We have significantly improved upon ELISpot user-success rates through advances in plate/membrane technologies with our partners, including fluorescent applications, as well as optimized protocols to meet the common pitfalls of this assay. This webinar will focus on two key aspects of the assay that can greatly impact overall performance: choice of plate configuration and the role of apoptotic cells.

 

Speaker Bio
Dr. Amedeo Cappione
Senior Scientist
Technology Development Group
Dr. Amedeo Cappione is a Senior Scientist in the Technology Development Group with an emphasis on Cell Culture Workflow Tools and Applications. In this capacity, he works with Business Development and Marketing partners to identify and assess cutting-edge technological opportunities for collaborative development, licensing, or acquisition. Dr. Cappione has over 20 years of research and product development experience spanning all aspects of Cell and Molecular Biology with a specific focus in cell-based assays.