Custom qPCR Probes

Assay optimization and validation are essential, even when using assays that have been predesigned and commercially obtained. Optimization is required to ensure that the assay is as sensitive as is r...
Keywords: AGE, Amplification, Buffers, Detection methods, Digestions, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Polymorphisms, Purification, RNA purification, Separation, Size-exclusion chromatography, Transcription

Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore contributes to an overall increase in background bec...
Keywords: Absorption, High performance liquid chromatography, Infrared spectroscopy, Polymerase chain reaction - quantitative

Sigma® is pleased to offer a tracking system for your custom oligo Blanket Purchase Orders. Our tracking system provides easy management of expenditures against your Blanket Purchase Order (P.O.). Si...

In North America alone, approximately eight million volunteers donate blood each year (estimate from the American Association of Blood Banks, see www.aabb.org/content). To ensure the safety of this b...
Keywords: Diseases, Infectious Diseases, Polymerase chain reaction - quantitative

There is a wide selection of possible custom probes that can be used for qPCR, and each has advantages for different applications. The summary below highlights the applications of commonly used custo...
Keywords: Extinction coefficient, Gene expression, High performance liquid chromatography, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative

Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing. Opposite of biosynthesis,...
Keywords: Capillary electrophoresis, Coupling reactions, Eliminations, Oxidations, Polymerase chain reaction, Polymerization reactions, Purification

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system. For some applications, a qPCR wil...
Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

Parasitic infection resulting from human acquisition of trematodes has been a significant health issue for centuries, and it continues to be an issue to this day. Trematodes are also commonly referre...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.
Keywords: Gene expression, High performance liquid chromatography, Mass spectrometry, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction - quantitative, Purification

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.
Keywords: Gene expression, High performance liquid chromatography, Mass spectrometry, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification

Locked Nucleic Acid in oligonucleotides other than qPCR probes is available in all countries except for the United States.
Keywords: Buffers, Gas chromatography, Gene expression, Melting, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Substitutions

The potential applications for quantitative real-time PCR (qPCR) have increased exponentially since the first description (Higuchi, 1993). However, researchers have been frustrated by complications s...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
Keywords: Gene expression, High performance liquid chromatography, Mass spectrometry, Nucleic acid hybridization, Polymerase chain reaction - quantitative, Purification

When using PCR (polymerase chain reaction) for detection of a target nucleic acid sequence, whether by qPCR (quantitative real-time PCR), dPCR (digital PCR), or derivative technology, numerous contro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

For more complex and demanding applications, utilize our consultative service to ensure the success of your assay. With personal consultation from our expert, technical support scientists, your reque...
Keywords: Gene expression, In Situ hybridization, Melting, Methylations, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative

For routine needs, improve your assay with our OligoArchitect Online design tool powered by the industry standard Beacon Designer™ (PREMIER Biosoft). The user-friendly interface utilizes the latest a...
Keywords: Gene expression, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative

We are pleased to offer OligoArchitect for all of your primer and probe design requirements. OligoArchitect is complimentary and includes both our online design tool and our consultative service.

We are pleased to offer OligoEvaluator, our online oligonucleotide sequence calculator that provides oligo analysis values.
Keywords: Melting

DNA Oligonucleotides are relatively stable molecules (Table 1). However, they require certain handling and storage techniques to ensure trouble-free experiments and maximize shelf life, respectively.
Keywords: Buffers, Degradations, Phase transitions, Polymerase chain reaction, Reductions

‘Black Hole Quencher,’ ‘BHQ-0,’ ‘BHQ-1,’ BHQ-2,’ and ‘BHQ-3’ are registered trademarks of Biosearch Technologies, Inc., Novato, California, U.S.A. (‘BTI’) Patents are currently pending for the BHQ te...
Keywords: Clinical, Diagnostic, Polymerase chain reaction, Veterinary

During DNA manufacturing, each nucleotide is coupled sequentially to the growing chain via phosphoramidite chemistry. In each coupling cycle, a low percentage of the oligonucleotide chains do not ext...
Keywords: Chromatography, Cloning, Electrophoresis, Filtration, Gas chromatography, Gel electrophoresis, High performance liquid chromatography, Microarray Analysis, Nuclear magnetic resonance spectroscopy, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Reversed-phase chromatography, Separation, Sequencing, Solvents

Key components of our QMS (quality management system) include: certificates of analysis; document control; change notification; vendor management; business agreements; and, corrective and preventive ...
Keywords: High performance liquid chromatography, Melting

Our ability to guarantee performance is directly related to our comprehensive understanding of oligonucleotide chemistry and synthesis platforms, our analytical systems and our experience in methods ...
Keywords: Chromatography, Diagnostic, Electrophoresis, Environmental, Extinction coefficient, Gel electrophoresis, High performance liquid chromatography, Karl Fischer titrations, Mass spectrometry, PAGE, Phase transitions, Reversed-phase chromatography, Solvents, Spectroscopy, Titrations

Our different manufacturing sites may use PAGE (polyacrylamide gel electrophoresis) to analyze duplexes depending on the nature of the sequences. PAGE separates duplexes on the basis of molecular wei...
Keywords: Electrophoresis, Gel electrophoresis, PAGE, Separation, Transfection

What is the molecular weight of OQ™? The molecular weights of OQ™A, OQ™B, OQ™C, and OQ™D are 518.6, 589.6, 582.6, and 650.7, respectively.

We offer Onyx Quencher (OQ™), a proprietary quencher for probed-based qPCR. Similar to other dark quenchers, OQ™ emits heat instead of light and therefore offers an improved signal-to-noise ratio com...
Keywords: Absorption, Polymerase chain reaction - quantitative

Guo Y, Ye JY, Divin C, Huang B, Thomas TP, Baker JR, Jr., & Norris TB (2010). Real-time biomolecular binding detection using a sensitive photonic crystal biosensor. Anal Chem, 82, 5211-5218. Biotin-l...
Keywords: Arylations, Cell proliferation, Cross couplings, DNA replication, Diagnostic, Gene expression, Mass spectrometry, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Vitamins

This protocol is for conjugating NHS-ester modifications, such as TxRd (Sulforhodamine 101-X) (protocol is effective for most NHS-ester modifications), to an oligonucleotide with an amino label, such...
Keywords: Bacterial conjugations, Buffers, Evaporation, High performance liquid chromatography, Precipitation, Purification

Scorpions probes are highly sensitive, sequence-specific, bi-labeled fluorescent probe/primer hybrids designed for qPCR.
Keywords: Gene expression, High performance liquid chromatography, Mass spectrometry, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification

As part of our customer education program, we have provided two recorded seminar series covering the topics of qPCR and MIQE. The recorded sessions are intended to provide a high-level overview of th...
Keywords: Polymerase chain reaction - quantitative

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative