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1α,25-Dihydroxyvitamin D3 solution

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

  • CAS Number 32222-06-3

  • Empirical Formula (Hill Notation) C27H44O3

  • Molecular Weight 416.64

  •  EC Number 200-578-6

  •  NACRES NA.24

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Properties

Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Biomarkers and Metabolomics Standards, Certified Reference Materials (CRMs),
Quality Level   300
grade   certified reference material
feature   Snap-N-Spike®/Snap-N-Shoot®
packaging   ampule of 1 mL
mfr. no.   Cerilliant®
concentration   5 μg/mL in ethanol
format   single component solution
storage temp.   −70°C
SMILES string   CC(CCCC(C)(C)O)C1CCC2C(\CCCC12C)=C\C=C3\CC(O)CC(O)C3=C
InChI   1S/C27H44O3/c1-18(8-6-14-26(3,4)30)23-12-13-24-20(9-7-15-27(23,24)5)10-11-21-16-22(28)17-25(29)19(21)2/h10-11,18,22-25,28-30H,2,6-9,12-17H2,1,3-5H3/b20-10+,21-11-
InChI key   GMRQFYUYWCNGIN-KWJBEKLWSA-N

Description

General description

1α,25-Dihydroxyvitamin D3 is a biologically active form of vitamin D3 in calcium absorption and deposition. It has widespread effects on cellular differentiation and proliferation, and can modulate immune responsiveness, and central nervous system function. Recent studies suggest that 1α,25-dihydroxyvitamin D3 acts as a chemopreventive agent against several malignancies including cancers of the prostate and colon and shows synergy with other anticancer compounds.

A Certified Spiking Solution® suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS Vitamin D assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.

Application

1α,25-Dihydroxyvitamin D3 may be used as a certified reference material for the determination of the analyte in biological fluids by liquid chromatography-tandem mass spectrometry.

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation

CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Cerilliant Corporation

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN1170 - class 3 - PG 2 - Ethanol, solution
WGK Germany 
WGK 1
Flash Point(F) 
57.2 °F - closed cup
Flash Point(C) 
14 °C - closed cup

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Hormone Standards

Over the past 17 years, hormone testing has increased significantly in the clinical laboratory setting as advances in the specificity and selectivity of LC-MS/MS technology have enabled hormones to s...
Keywords: Clinical, Clinical Chemistry, Diagnostic, Diseases, Endocrinology, High performance liquid chromatography, Hormones, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.1 This heightened interest has spawned the need for analytical strategies to assess an ...
Craig Aurand
Reporter US Vol 31.1
Keywords: Clinical, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Metabolites, Precipitation, Sample preparations, Separation, Solid phase extractions, Solvents, Vitamins

LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

The unique combination of column selectivity using Ascentis® Express F5 and phospholipid removal using HybridSPE®-PLus provides a robust and accurate LC-MS method for accurate and sensitive analysis ...
Craig Aurand, Strategic Marketing Team Manager, Integrated Sample Preparation and Hugh Cramer, Scientist, Advanced Sample Preparation Technologies
Keywords: Buffers, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Reductions, Sample preparations, Separation, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents, Vitamins

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

The unique combination of column selectivity using Ascentis Express F5 and phospholipid removal using HybridSPE-PLus provides a robust and accurate LC-MS method for accurate and sensitive analysis of...
Craig Aurand, Hugh Cramer
Reporter US Volume 32.1
Keywords: Buffers, Enzyme-linked immunosorbent assay, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Reductions, Sample preparations, Separation, Solvents, Vitamins

Snap-N-Spike® reference solutions of 25-Hydroxyvitamin D

Over the last decade, vitamin D testing has increased significantly in the clinical laboratory setting as research studies have linked vitamin D deficiency to numerous diseases from diabetes, heart d...
Keywords: Clinical, Diabetes, Diseases, High performance liquid chromatography, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Vitamins

Vitamins | Technical Article

Sigma-Aldrich's Cerilliant brand provides certified Snap-N-Spike® | Snap-N-Shoot® solutions of numerous vitamins from the B, A, K and E groups to vitamin D including 25-Hydroxy and 1,25-Dihydroxy ana...
Keywords: Clinical, Degradations, Vitamins

Peer-Reviewed Papers
15

References

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