25-Hydroxyvitamin D2 solution H087

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Properties

Related Categories	Additional Standards, Analytical Standards, Analytical/Chromatography, Biomarkers and Metabolomics Standards, Certified Reference Materials (CRMs), Certified Reference Materials from Cerilliant, Hydroxyvitamin D Standards, Pharmaceuticals, Illicit Drugs & Alcohol, Steroids/Hormones, Vitamins More...
Quality Level	300
grade	certified reference material
feature	Snap-N-Spike^®/Snap-N-Shoot^®
packaging	ampule of 1 mL
mfr. no.	Cerilliant^®
concentration	5 μg/mL in ethanol
format	single component solution
storage temp.	−70°C
SMILES string	CCCC(O)CC.CC(\C=C\C(C)C(C)(C)O)C1CCC2C(CCCC12C)\C=C\C=C/C=C
InChI	1S/C28H44O2/c1-19-10-14-24(29)18-23(19)13-12-22-8-7-17-28(6)25(15-16-26(22)28)20(2)9-11-21(3)27(4,5)30/h9,11-13,20-21,24-26,29-30H,1,7-8,10,14-18H2,2-6H3/b11-9+,22-12+,23-13-/t20-,21+,24+,25-,26+,28-/m1/s1
InChI key	KJKIIUAXZGLUND-ICCVIKJNSA-N

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Description

General description

A Certified Spiking Solution^® of 25-Hydroxyvitamin D2, a prohormone whose blood serum levels are used to determine Vitamin D nutritional status. This solution standard is suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ.

The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike^® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.

Application

25-Hydroxyvitamin D2 may be used as a certified reference material (CRM) for the determination of the analyte in biological samples by chromatography techniques.

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation

CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Cerilliant Corporation

Safety & Documentation

Safety Information

Symbol

GHS02,GHS07

Signal word

Danger

Hazard statements

H225 - H319

Precautionary statements

P210 - P305 + P351 + P338

RIDADR

UN1170 - class 3 - PG 2 - Ethanol, solution

WGK Germany

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation

Rat serum was spiked at 300 ng/mL with 25-hydroxyvitamin D2, 25- hydroxyvitamin D3 and epi-25-hydroxyvitamin D3. Protein precipitation was performed offline by adding 100 μL of spiked serum into a 50...
Craig Aurand¹, David Bell¹, Anders Fridstrom², and Klaus Buckendahl²
¹Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA ²Sigma-Aldrich Chemie GmbH, 82024 Taufkirchen, Germany
Keywords: Absorption, Detection methods, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Precipitation, Sample preparations, Separation, Solvents, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

Hormone Standards

Over the past 17 years, hormone testing has increased significantly in the clinical laboratory setting as advances in the specificity and selectivity of LC-MS/MS technology have enabled hormones to s...
Keywords: Clinical, Clinical Chemistry, Diagnostic, Diseases, Endocrinology, High performance liquid chromatography, Hormones, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.1 This heightened interest has spawned the need for analytical strategies to assess an ...
Craig Aurand
Reporter US Vol 31.1
Keywords: Clinical, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Metabolites, Precipitation, Sample preparations, Separation, Solid phase extractions, Solvents, Vitamins

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents, Vitamins

LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

From our library of Articles, Sigma-Aldrich presents LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Solid phase extractions, Vitamins

Mitigation of Deuterium Scrambling in Stable-Labeled Internal Standards during LC-MS MS Analysis

Improvements in LC-MS/MS sensitivity and multiple reaction monitoring (MRM) capabilities are resulting in further adoption of LC-MS/MS technology in clinical settings. One specific clinical challenge...
Authors: Joshua Cooper¹, Billy Molloy², Huahua Jian¹, Lisa Calton², Derrell Johnson¹, Isil Dilek¹, Uma Sreenivasan¹, Don Cooper²
¹Cerilliant Corporation, 811 Paloma Drive, Suite A, Round Rock, TX 78665 ² Waters Corporation, Atlas Park, Simonsway, Manchester, M22 5 PP UKCONCLUSIONS
Keywords: Clinical, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Nuclear magnetic resonance spectroscopy, Phase transitions

Snap-N-Spike® reference solutions of 25-Hydroxyvitamin D

Over the last decade, vitamin D testing has increased significantly in the clinical laboratory setting as research studies have linked vitamin D deficiency to numerous diseases from diabetes, heart d...
Keywords: Clinical, Diabetes, Diseases, High performance liquid chromatography, Ion-exchange chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Vitamins

Vitamins | Technical Article

Sigma-Aldrich's Cerilliant brand provides certified Snap-N-Spike® | Snap-N-Shoot® solutions of numerous vitamins from the B, A, K and E groups to vitamin D including 25-Hydroxy and 1,25-Dihydroxy ana...
Keywords: Clinical, Degradations, Vitamins

Peer-Reviewed Papers

15

References

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