Ascentis® Express U/HPLC Columns

 Maximum speed and resolution in HPLC and UHPLC

The Fused-Core® particle technology behind Ascentis® Express columns delivers maximum speed and efficiency on both HPLC and UHPLC systems. The novel superficially porous silica particles (SPP) are available in three different particle sizes and two different pore sizes for optimal suitability from micro over UHPLC and HPLC to semi-preparative use:


Fused-Core® particle size 2 µm

 Best Fused-Core® UHPLC column

An optimized solution for high-throughput small molecule analysis.

Fused-Core® particle size 2.7 µm


 Fast HPLC on any system

A practical solution that delivers UHPLC performance from any HPLC.

Fused-Core® particle size 5 µm


 The lab work-horse column

True plug and play solution for improving existing 3 or 5 μm fully porous particle HPLC columns.


A wide range of column chemistries enable the superior separation of several compound classes, meeting the demands in many different application fields:

Bonded Phase Chemistry USP Designation Chromatographic Properties / Use Particle Size (s) (µm) Pore Size (Å)
C30 Triacontyldimethyl L62 Excellent selectivity for very hydrophobic compounds, long-chain and structurally related isomers 2.7 160
C18 Dimethyloctadecyl L1 Outstanding performance for a broad range of analytes 2, 2.7, 5 90
Peptide ES-C18 Diisobutyloctadecyl L1 Fast separation of peptides and polypeptides with high peak capacity 2.7 160
AQ-C18 Polar modified Octadecyl L1 Resistant to dewetting; compatible to 100% aqueous mobile phase 2, 2.7, 5 90
C8 Dimethyloctyl L7 Enhanced retention for less hydrophobic compounds or faster separation if retention on C18 is too long 2, 2.7, 5 90
RP-Amide C16-Amide L60 Complementary selectivity to alkyl phases 2, 2.7, 5 90
Phenyl-Hexyl Dimethylphenyl-hexyl L11 Enhanced selectivity for aromatic compounds; strong pi-pi donor 2, 2.7, 5 90 and 160
Biphenyl Dimethylbiphenyl L11 Enhanced selectivity for aromatic compounds 2, 2.7, 5 90
F5 (PFP) Pentafluorophenylpropyl L43 Outstanding selectivity for stereoisomers, strong pi-pi acceptor 2, 2.7, 5 90
ES-Cyano Diisopropylcyanopropyl L10 Enhanced retention for polar compounds and much less retention for hydrophobic compounds 2, 2.7, 5 90
OH5 Penta-hydroxy L95 Ideal for the separation of very polar compounds with a LogP value close to 0 or less than 0 2, 2.7, 5 90
HILIC bare silica L3 Enhanced separation of polar compounds; can be used in HILIC and normal-phase mode. 2, 2.7, 5 90