Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of protein-protein interactions with flow cytometry readouts a reality. With the enhanced sensitivity of the Duolink® flowPLA Detection Kit, detection of low-abundant protein targets in cell populations is now possible. This easy-to-follow protocol works with any flow cytometry instrument to achieve publication-ready results.

The Duolink® flowPLA detection kits provide enough material to perform ~40 reactions, each with 100,000 cells in 100 µL reaction volume. Each kit contains:

  • Ligation Stock (5x) – Diluted to make 1x Ligation Buffer
  • Ligase (1 U/μL) – Added to make the Ligation Solution
  • Amplification Stock (5x) – Diluted to make 1x Amplification Buffer
  • Polymerase (10 U/μL) – Added to make the Amplification Solution
  • Detection Stock (5x) – Diluted to make 1x Detection Buffer
Blood Mononuclear Cells using Duolink®

Figure 1.Detection of Post-translational Modifications in Peripheral Blood Mononuclear Cells using Duolink® flowPLA

Low-abundant Protein-Protein Interactions using Duolink® flowPLA

Figure 2.Detection of Low-abundant Protein-Protein Interactions using Duolink® flowPLA

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