Anti-biofilm efficacy of nitric oxide-releasing silica nanoparticles.

Biomaterials (2009-02-24)
Evan M Hetrick, Jae Ho Shin, Heather S Paul, Mark H Schoenfisch
ABSTRACT

The ability of nitric oxide (NO)-releasing silica nanoparticles to kill biofilm-based microbial cells is reported. Biofilms of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans were formed in vitro and exposed to NO-releasing silica nanoparticles. Replicative viability experiments revealed that >or= 99% of cells from each type of biofilm were killed via NO release, with the greatest efficacy (>or= 99.999% killing) against gram-negative P. aeruginosa and E. coli biofilms. Cytotoxicity testing demonstrated that the highest dose of NO-releasing silica nanoparticles inhibited fibroblast proliferation to a lesser extent than clinical concentrations of currently administered antiseptics (e.g., chlorhexidine) with proven wound-healing benefits. This study demonstrates the promise of employing nanoparticles for delivering an antimicrobial agent to microbial biofilms.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetraethyl orthosilicate, reagent grade, 98%
Sigma-Aldrich
Tetraethyl orthosilicate, ≥99.0% (GC)
Sigma-Aldrich
Tetraethyl orthosilicate, 99.999% trace metals basis
Sigma-Aldrich
Tetraethyl orthosilicate, packaged for use in deposition systems

Social Media

LinkedIn icon
Twitter icon
Facebook Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.