R8758

Sigma-Aldrich

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
L-glutamine: yes
phenol red: yes
HEPES: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.

Application

RPMI-1640 Medium has been used for maintaining cell line medium and serves as a medium for different cells.
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Does Product R8758, RPMI-1640 Medium, contain glucose?

    RPMI contains glucose at 2 g/L.  The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.

  2. Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?

    This formulation of RPMI already contains L-glutamine at 0.3 g/L.  It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).

  3. Does Product R8758, RPMI-1640 Medium, contain HEPES?

    There is no HEPES in this formulation of RPMI.  We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

  4. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

E L Davies et al.
Clinical and experimental immunology, 145(1), 183-189 (2006-06-24)
An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70...
Thaneas Prabakaran et al.
PloS one, 6(9), e25065-e25065 (2011-09-29)
Injury to the glomerular podocyte is a key mechanism in human glomerular disease and podocyte repair is an important therapeutic target. In Fabry disease, podocyte injury is caused by the intracellular accumulation of globotriaosylceramide. This study identifies in the human...
Anders Abildgaard et al.
Psychoneuroendocrinology, 79, 40-48 (2017-03-05)
The gut microbiota has recently emerged as an important regulator of brain physiology and behaviour in animals, and ingestion of certain bacteria (probiotics) therefore appear to be a potential treatment for major depressive disorder (MDD). However, some conceptual and mechanistical...
Ryota Kurimoto et al.
International journal of oncology, 48(5), 1825-1836 (2016-03-18)
Epithelial-to-mesenchymal transition (EMT) is a malignant cancer phenotype characterized by augmented invasion and metastasis, chemoresistance, and escape from host-immunity. This study sought to identify efficient methods for inducing EMT reversion, to evaluate alterations in chemosensitivity and immune-protectiveness, and to elucidate...
Kaja Lund et al.
Journal of experimental & clinical cancer research : CR, 36(1), 187-187 (2017-12-21)
Development of resistance to 5-fluorouracil (5-FU) is a major problem in treatment of various cancers including pancreatic cancer. In this study, we reveal important resistance mechanisms and photochemical strategies to overcome 5-FU resistance in pancreatic adenocarcinoma. 5-FU resistant (5-FUR), epithelial-to-mesenchymal-like...
Articles
MISSION® Target ID Library for Human miRNA Target Identification and Discovery;
Read More
Protocols
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.