lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
n20/D 1.468 (lit.)
0.06 mM (20-25°C)
cloud point 76 °C
1.095 g/mL at 25 °C (lit.)
complies for IR spectroscopy
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Increased temperature can lead to autoxidation of TWEEN 20 in solution. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. TWEEN 20 is heat sensitive; it will darken when exposed to elevated temperatures. Autoclaving is done at the customer's risk.
Origin information on any lot of Tween 20 is specific to that lot, and can be found on its Certificate of Origin (CofO), which may be accessed on the Sigma-Aldrich website under its specific product and lot number.
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